Composite

Part:BBa_K5498015

Designed by: Enyu LI   Group: iGEM24_jky-wuhan-china   (2024-09-30)


pfago

Recombinant vector pET-23a-PfAgo mainly consists of strong promoter T7, RBS(ribosomebinding site), PfAgo gene and T7 terminator. In order to facilitate the expression of purified PfAgo protein in E. coli, a 6×His tag was fused to the 5 'end of the PfAgo gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 816
    Illegal EcoRI site found at 2406
    Illegal XbaI site found at 47
    Illegal PstI site found at 228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 816
    Illegal EcoRI site found at 2406
    Illegal PstI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 816
    Illegal EcoRI site found at 2406
    Illegal BglII site found at 1472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 816
    Illegal EcoRI site found at 2406
    Illegal XbaI site found at 47
    Illegal PstI site found at 228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 816
    Illegal EcoRI site found at 2406
    Illegal XbaI site found at 47
    Illegal PstI site found at 228
    Illegal AgeI site found at 2345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 28


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